Bound Water Dynamics and Phase Change Thermal Engineering Lab.
PUBLICATIONS
1. Original Paper (full paper with peer Review)
- 2) Ryo Shirakashi and Ichiro Tanasawa, Numerical Simulation of Freezing Process of Biological Tissues, HEAT TRANSFER-JAPANESE RESEARCH vol.24 No.4.(1995), 342-355.
- 8) Minoru Hosoda, Shigefumi Nishio, Ryo Shirakashi, STUDY OF MEANDERING CLOSED-LOOP HEAT-TRANSPORT DEVICE (VAPOR-PLUG PROPAGATION PHENOMENA), JSME International Journal vol.42, No.4 Series B (1999), 737-744.
- 9) Ryo Shirakashi and Ichiro Tanasawa, Measurement of Critical Osmotic Pressure for Biological Cells (Minimum Cell-Volume-Ratio as an Estimation of Viability), JSME International Journal vol.42, No.3 Series B (1999), 561-565.
- 10) Ryo Shirakashi and Ichiro Tanasawa, The Method of Designing Prefreezing and Freezing Process of Biological Tissues, JSME International Journal vol.42, No.3 Series C (1999), 790-795
- 15) Ryo Shirakashi, C.M.Kostner, K.J. Muller, M.Kurschner, U.Zimmermann and V.Sukhorukov, Intracellular Delivery of Trehalose into Mammalian Cells by Electropermeabilization, Journal of Membrane Biology vol.189 (2002), 45-54.
- 16) Shigefumi Nishio, Shin-ichi Nagata, Shohei Numata and Ryo Shirakashi, Study on Thermal Characteristics of Bubble-Driven Heat-Transport Device, Heat Transfer - Asian Research, 32 (2), (2003), 167-177.
- 18) Ryo Shirakashi, Killian J. Muller, Vladimir L. Sukhorukov and Ulrich Zimmermann, Effects of Physiological Isotonic Cryoprotectants on Living Cells During the Freezing-Thawing Process and Effects of Their Uptake by Electroporation: Sp2 Cells in Alginate - Trehalose Solutions, Heat Transfer - Asian Research, 32 (6), (2003), 511-523.
- 20) Ryo Shirakashi, Vladimir L. Sukhorukov, Ichiro Tanasawa and Ulrich Zimmermann, Measurement of the permeability and resealing time constant of the electroporated mammalian cell membranes, International Journal of Heat and Mass Transfer 47(21), (2004), 4517-4524.
- 21) Randolph Reuss, Jorg Ludwig, Ryo Shirakashi, Friederike Ehrhart, Heiko Zimmermann, Stephan Schneider, Matthias M. Weber, Ulrich Zimmermann, Heike Schneider, and Vladimir L.Sukhorukov, Intracellular delivery of carbohydrates into mammalian cells through swelling-activated pathways, Journal of Membrane Biology vol.200 (2004), 67-81.
- 27) V. L. Sukhorukov J. M. Endter, D. Zimmermann, R. Shirakashi, S. Fehrmann, M. Kiesel, R. Reuss, D. Becker, R. Hedrich, E. Bamberg, Th. Roitsch and U. Zimmermann, Mechanisms of Electrically Mediated Cytosolic Ca2+ Transients in Aequorin-Transformed Tobacco Cells, Biophysical Journal Vol.93 (2007), 3324-3337.
- 28) R. Shirakashi, K. Kasahara, S. Miura, K. Tsuyuki and D. Tajima, Dielectric Spectroscopy and Relaxation Analysis of Methane Hydrate, High Temperatures High Pressures, Vol.37, No.1 (2008), 71-80.
- 29) C.Provin, K.Takanob, Y.Sakai, T.Fujii, R. Shirakashi, A method for the design of 3D scaffolds for high-density cell attachment and determine of optimum perfusion culture conditions, Journal of Biomechanics, Vol.41, No.7 (2008), 1436-1449.
- 34) Ryo Shirakashi, Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov, and Ulrich Zimmermann, Evaluation of FEM Electric Field Analysis Using the Electric Properties of Giant Unilamellar Vesicles (GUV) and Jurkat Cells Measured by Electrorotation Spectroscopy : Introduction of Cryo- and Lyoprotectants by Electrofusion of GUVs and Living Cells, Heat Transfer Asian Research, Vol. 38 (3) (2009), 168-182.
- 35) C.Provin, K.Takano, T. Yoshida, Y. Sakai, T. Fujii, R.Shirakashi, Low O2 metabolism of HepG2 cells cultured at high density in a 3D microstructured scaffold, Biomedical Microdevices, Vol.2 Issue 11 (2009), 485-494.
- 36) Vladimir L. Sukhorukov, Dennis Imes, Michael W. Woellhaf, Joseph Andronic, Martin Kiesel, Ryo Shirakashi, Ulrich Zimmermann, Heiko Zimmermann, Pore size of swelling-activated channels for organic osmolytes in Jurkat lymphocytes, probed by differential polymer exclusion, Biochemica et Biophysica Acta (BBA) - Biomemebranes, Vol.1788 (2009), 1841-1850
- 37) R. Shirakashi, I. Hagiya, S. Nishio, Study on high performance evaporator with micro-grooves (Measurement of capillary force in a single groove and modeling of heat and mass transfer), Heat Transfer - Asian Research, Vol.38(6) (2009), 331-346.
- 38) S.Ueno, R. Shirakashi, K. Kudoh, T.Higuchi, G. Do, T.Araki, Y.Sagara, Effect of the grain boundary of ice crystals in a frozen gelatin solution on the dielectric properties at a subzero temperature, Bioscience Biotechnology and Biochemistry, Vol.73(11), (2009), 2478-2482
- 41) Ryo SHIRAKASHI, Vladimir L. SUKHORUKOV, Randolph REUSS, Alexander SCHULZ, Ulrich ZIMMERMANN, Effects of a Pulse Electric Field on Electrofusion of Giant Unilamellar Vesicle (GUV)-Jurkat Cell (Measurement of Fusion Ratio and Electric Field Analysis of Pulsed GUV-Jurkat Cell) , Journal of Thermal Science and Technology Vol. 7, No. 4 (2012), 589-602.
- 43) Ryo Shirakashi, Miriam Mischke, Peter Fischer, Simon Memmel, Georg Krohne, Gunter R. Fuhr, Heiko Zimmermann, Vladimir L. Sukhorukov, Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation, Biochemical and Biophysical Research Communications, Vol. 428 (2012), 127-131.
- 44) Shigenao Maruyama, Koji Deguchi, Masazumi Chisaki, Junnosuke Okajima, Atsuki Komiya, Ryo Shirakashi, Proposal for a low CO2 emission power generation system utilizing oceanic methane hydrate, Energy 47 (2012) 340-347.
- 45) Ryo Shirakashi, Tsuyoshi Ogawa, Jun Yamada, Dielectric spectroscopy of thin surface layer by Differential Time Domain Reflectometry using a Coplanar Waveguide Circuit line probe, Measurement Science and Technology, Vol. 24, No.2 (2013) 025501+10.
- 47) Yanxin Hu, Jiang Cheng, Wei Zhang, Ryo Shirakashi, Shuangfeng Wang, Thermal performance enhancement of grooved heat pipes with inner surface treatment, International Journal of Heat and Mass Transfer, Vol. 67 (2013) 416-419.
- 48) Zirong Lin, Shuangfeng Wang, Ryo Shirakashi, L. Winston Zhang, Simulation of a miniature oscillating heat pipe in bottom heating mode using CFD with unsteady modeling, International Journal of Heat and Mass Transfer, Vol.57 (2013) 642-656.
- 50) Joseph Andronic, Ryo Shirakashi, Simone U. Pickel, Katherine M. Westerling, Teresa Klein, Thorge Holm, Markus Sauer, Vladimir L. Sukhorukov, Hypotonic activation of the myo-inositol transporter SLC5A3 in HEK293 cells probed by cell volumetry, confocal and super-resolution microscopy, PLOS ONE, DOI:10.1371, journal.pone.0119990 (2015)
- 51) Ryo Shirakashi, Tatsuo Yasui, Simon Memmel, Vladimir L. Sukhorukov, Electro-microinjection of fish eggs with an immobile capillary electrode, BIOMICROFLUIDICS 9, 064109 (2015)
- 52) Takao Furuki, Takahiro Watanabe, Tadaomi Furuta, Kiyoshi Takano, Ryo Shirakashi, Minoru Sakurai, The dry preservation of giant vesicles using a Group 3 LEA protein model peptide and its molecular mechanism, Bulletin of the Chemical Society of Japan, 89 (2016) 1493
- 53) Ryo Shirakashi, Yuki Amano, Jun Yamada, Measurement of the Water Relaxation Time of ε-Polylysine Aqueous Solutions, International Journal Thermophysics, DOI 10.1007/s10765-017-2213-y (2017) 38:75
- 54) Yukiko Otsuka, Ryo Shirakashi, Kazuhiko Hirakawa, Bound states of water in gelatin discriminated by near-infrared spectroscopy, Japanese Journal of Applied Physics 56, 111602 (2017) doi.org/10.7567/JJAP.56.111602
- 55) Ryo Shirakashi, Kiyoshi Takano, Recrystallization and Water Absorption Properties of Vitrified Trehalose Near Room Temperature, Pharmaceutical Research 35: 139 (2018) https://doi.org/10.1007/s11095-018-2420-7
- 56) Kiyoshi Takano, Ryo Shirakashi, Development of Dry-preservation technology for biological protein at room temperature - measurement of glass transition temperature and residual activity of lactate dehydrogenase -, Transaction of the Japan Society of Refregerating and Air Conditioning Engineers Vol.35, No.4, 325-330 (2018)
- 57) Yuan Pang, Stephanie Sutoko, Yohei Horimoto, Ding Weng, Kevin Montagne, Kikuo Komori, Kiyoshi Takano, Ryo Shirakashi, Masahiro Anzai, Toshiki Niino,Yasuyuki Sakai, Biodegradable and hollowed micro-scaffolds for improved modularassembly-based tissue engineering: Design, 3D fabrication, and feasibility inrandomly packed perfusion culture, Biochemical Engineering Journal, Vol. 149, 15 (2019) 107239
- 58) Sikai Wang, Ryo Shirakashi, Local Joule heating and electric force on biological membrane duringelectro-microinjection, International Journal of Heat and Mass Transfer 140 (2019) 798-806
- 59) Lin Wei, Ryo Shirakashi, Measurement of the diffusion coefficient and hydrogen bonds of water in a dry-protective solution by microscopic near-infrared spectroscopy, Food and Bioproducts Processing, vol. 124, 97-106 (2020)
- 60) Lin Wei, Ryo Shirakashi, Effect of Relaxation Times in a Preservative Solution on Protein Deterioration Rate, Journal of physical Chemistry B, 124, 8741-8749(2020)
- 61) Lin Wei, Ryo Shirakashi, Simulation of air/vacuum desiccation process for high-quality preservation of proteins, Journal of Food Process Engineering, DOI: 10.1111/jfpe.13962 (2022)
- 62) Hiroaki Matsuura and Ryo Shirakashi, Exclusion of DC conductivity effect from dielectric loss spectrum using Kramers-Kronig relations for evaluation of slow dynamics of water molecules, Japanese Journal of Applied Physics 61, 068003 (2022)
- 63) Junkai Zhang, Hiroaki Matsuura, Ryo Shirakashi, Prediction of Water Relaxation Time Using Near Infrared Spectroscopy, Journal of Food Process Engineering, e14095, https://doi.org/10.1111/jfpe.14095 (2022)
- 64) Kang Hu, Hiroaki Matsuura, Ryo Shirakashi, Stochastic Analysis of Molecular Dynamics Reveals the Rotation Dynamics Distribution of Water around Lysozyme, The Journal of Physical Chemistry PartB SectionB, 126, 24, 4520-4530 (2022) https://doi.org/10.1021/acs.jpcb.2c00970
- 65) Ryo Shirakashi, Zisis Kozlakidis, Birendra Kumar Yadav, Wayne Ng, Jajah Fachiroh, Hanh Vu, Tatsuaki Tsuruyama, Koh Furuta, Decarbonization in Biobanking: A Potential New Scientific Area, BIOPRESERVATION AND BIOBANKING 20, 5, (2022) DOI: 10.1089/bio.2022.0146
- 66) Ryo Shirakashi, Dmitri Sisario, Danush Taban, Tessa Korsa, Sophia B. Wanner, Julia Neubauer, Cholpon S. Djuzenova, Heiko Zimmermann, Vladimir L. Sukhorukov, Contraction of the rigor actomyosin complex drives bulk hemoglobin expulsion from hemolyzing erythrocytes, Biomechanics and Modeling in Mechanobiology, (2022). https://doi.org/10.1007/s10237-022-01654-6
- 67) Philippa K Brosch, Tessa Korsa, Danush Taban, Patrick Eiring, Sascha Hildebrand, Julia Neubauer, Heiko Zimmermann, Markus Sauer, Ryo Shirakashi, Cholpon S Djuzenova, Dmitri Sisario, Vladimir L Sukhorukov, Glucose- and inositol-transporters, SLC5A1 and SLC5A3, in glioblastoma cell migration, Cancers, 14, 5794 (2022). https://doi.org/10.3390/cancers14235794
- 68) Kang Hu, Ryo Shirakashi, Dynamic Electric Field Alignment Determines the Water Rotational Motion around Protein, The Journal of Physical Chemistry PartB SectionB, 127, 6, 1376-1384 (2023), https://doi.org/10.1021/acs.jpcb.2c07405 (Feb)
- 69) Kang Hu, Ryo Shirakashi, Molecular dynamics study of water rotational relaxation in saccharide solution for the development of bioprotective agent, Journal of Molecular Liquids, 380,121707 (2023), https://doi.org/10.1016/j.molliq.2023.121707
- 70) Junkai Zhang, Hiroaki Matsuura, Ryo Shirakashi, A method for measuring dielectric relaxation of water by NIR spectroscopy: Applicability and application to measurement of water diffusion coefficient, Journal of Food Process Engineering, e14335, https://doi.org/10.1111/jfpe.14335 (April)
- 71) Hiroaki Matsuura, Kiyoshi Takano, Ryo Shirakashi, Slow water dynamics in dehydrated human Jurkat T cells evaluated by dielectric spectroscopy with Bruggeman-Hanai equation, RSC Advances, 13 (2023), 20934-20940, DOI: 10.1039/d3ra02892e
- 72) Junkai Zhang, Ryo Shirakashi, Measurement of the rotational relaxation time of intracellular water in dried yeast and Jurkat cells by near infrared spectroscopy, Biochemical and Biophysical Research Communications, Vol. 710 (2024), 149857, https://doi.org/10.1016/j.bbrc.2024.149857
- 73) Kang Hu, Ryo Shirakashi, Determination of the first hydration layer from the perspective of local electric field reorientation, Chemical Physics Letters, 845, 16 (2024), 141302, https://doi.org/10.1016/j.cplett.2024.141302
- 75) Kosei Kawai, Ryo Shirakashi, Water rotational relaxation time measurement by shortwave infrared micro spectroscopy(SWIR) at sub-zero temperatures, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 322 (2024) 124707, https://doi.org/10.1016/j.saa.2024.124707
- 76) Yuanheng Zhao, Hiroaki Matsuura, Ryo Shirakashi, Water rotational relaxation time of preservation solutions relates to storage lifespan of cells in isochoric cryopreservation system, Food and Bioproducts Processing 147 (2024) 199-208, https://doi.org/10.1016/j.fbp.2024.06.012
- 77) Masaaki Kitajima, Hirohisa Abe, Ryo Honda, Hiroyuki Kobayashi, Tomohiro Kuroita, Ayuko Nemoto, Ryo Shirakashi, Rodney Scott and Koh Furuta, Biobanking: Possibilities for Wastewater-Based Epidemiology, Biopreservation and Biobanking, Vol.23 (2025) https://doi.org/10.1089/bio.2024.0118
- 78) Hanh Vu, et al., Experts Speak Forum: Decarbonization for Green Biobanking—The Current Landscape and Challenges for the Future, Biopreservation and Biobanking, Vol.23 (2025) https://doi.org/10.1089/bio.2024.0128
2. International conference in full papers
- 1) Ryo Shirakashi and Ichiro Tanasawa, Measurement of diffusion coefficient of cryoprotectant mixture in biological tissues, 3rd KSME/JSME Thermal Engineering conference (1996), 267-272.
- 2) Ryo Shirakashi and Ichiro Tanasawa, Method of Designing Pre-freezing Protocol in Cryopreservation of Biological Materials, THE ANNALS OF NEW YORK ACADEMY OF SCIENCES, Biotransport: Heat and Mass Transfer in Living Systems, vol.858, (1998), 175-182.
- 3) Minoru Hosoda, Shigefumi Nishio, Ryo Shirakashi, MEANDERING CLOSED-LOOP HEAT-TRANSPORT TUBE (PROPAGATION PHENOMENA OF VAPOR PLUG), 5th ASME /JSME Joint Thermal Engineering Conference AJTE99-6306 (1999).
- 4) Xianglan BAI, Ryo SHIRAKASHI and Shigefumi NISHIO, ON THE MECHANISM OF AGING EFFECTS ON THE DIELECTRIC PROPERTIES OF POLYCRYSTALLINE ICE, Proceedings of the 4th JSME-KSME Thermal Engineering Conference, vol.3, (2000), 325-330.
- 5) Tetsuya Araki, Ryo Shirakashi and Yasuyuki Sagara, Numerical Simulation of Freezing Process of Coffee Solution Related to Freeze-Drying, Proceedings of 2nd Asian-Oceania Drying Conference, (2001), 155-163.
- 6) S.Nishio, S.Nagata, S.Baba and R.Shirakashi, Thermal performance of SEMOS heat pipes, 12th International Heat Transfer Conference vol.4 (2002), 477-482.
- 7) R.Shirakashi, BAI Xianglan and S.Nishio, Dielectric Property of Ice for Rapid and Uniform Dielectric Heating, The 6th ASME-JSME Thermal Engineering Joint Conference AJTC 2003.
- 8) K.KAMINISHI, T.ARAKI, R.SHIRAKASHI, Y.SAGARA, THREE-PHASE MODEL FOR SIMULATING FREEZING PROCESS OF AQUEOUS SOLUTIONS, Inter National Congress of Refrigeration ICR0232 (2003).
- 9) R.Shirakashi, The long term preservation of living biomaterials, Proceedings of the 3rd Korea-Japan Joint Seminar on Heat and Mass Transfer (2005), 17-25.
- 10) C. Provin, K. Takano, R. Shirakashi, Y. Sakai, T. Fujii, Fabrication of Microbioreactors with an Optimized Structure Designed for High Density Culture of Hepatocyte, 2006 Inernatinal Conference on Microtechnologies in Medicine and Biology (2006), 267-270.
- 11) Ryo Shirakashi, Tomomi Yoshida, Christophe Provin, Kiyoshi Takano,Yasuyuki Sakai, Teruo Fujii, Steady measurement of glucose metabolism of hepatocyte, ASME-JSME Thermal Engineering and Summer Heat Transfer Conference, HT2007-32750 (2007).
- 12) Isao HAGIYA, Shigefumi NISHIO, Ryo SHIRAKASHI, Heat and Mass Transfer Characteristics of Single Micro Groove for Micro Groove Evaporator, The 3rd International Conference on cooling and Heating Technology (2007) 34-38.
- 13) M. Maruyama, M. Chisaki, A. Komiya and R.Shirakashi, A proposal of low CO2 emission power generation system utilizing oceanic methane hydrate, Proceedings of the International Conference on Power Engineering-09, (2009) 2-343-2-348.
- 14) Ryo Shirakashi, Kiyoshi Takano, Christophe Provin, Yasuyuki Sakai, Teruo Fujii, Measurement of Cellular Adhesion Ratios under High Shear Flow on Various Substrates, Proceedings of the 8th ASME-JSME Thermal Engineering Joint Conference, (2011), AJTEC2011-44404.
3. Conference Abstracts
- 1) Ryo Shirakashi and Ichiro Tanasawa, Numerical Simulation of Freezing Process of Biological Tissues, The 31st Annual Meeting of The Society for Cryobiology (1994).
- 13) Ryo Shirakashi and Ichiro Tanasawa, MEASUREMENT OF MEMBRANE PERMEABILITY OF ENDOTHELIAL CELL, Third World Congress of Biomechanics ABSTRACTS, (1998), 181.
- 25) Ryo Shirakashi and Yasuyuki Sakai, Enhancement of membrane permeability by electroporation, The 37st Annual Meeting of The Society for Cryobiology (2000).
- 26) Ryo Shirakashi, Xianglang Bai and Shigefumi Nishio, Measurement of dielectric-loss spectrum of ice for rapid-uniform thawing, The 37st Annual Meeting of The Society for Cryobiology (2000).
- 30) Tetsuya Araki, Ryo Shirakashi and Yasuyuki Sagara, A Numerical Model for Freezing of Coffee Solutions, Proceedings of 7th Conference of Food Engineering - AIChE Annual Meeting, (2001).
- 40) Shigefumi Nishio, Takeshi Iwakami, Ryo Shirkashi and Makoto Mita, Study of Micro-Miniture JT Cooler, Thermal Science and Engineering, vol.11, No.4 (2003), 29-30.
- 41) Ryo Shirakashi, Kilian J. Muller, Vladimir L. Sukhorukov, Ichiro Tanasawa and Ulrich Zimmermann, Effects of Saccharide on Living Cells during the Freezing-Thawing Process, Cryobiomol 2003.
- 49) Ryo Shirakashi, Effect of saccharide on intracellular ice formation, cryomedicine2004 Main Program and Abstracts, (2004), 28.
- 50) Randolph Reuss, Ryo Shirakashi, Friederike Ehrhart, Heiko Zimmermann, Ulrich Zimmermann, Heike Schneider, and Vladimir L.Sukhorukov, Introduction of carbohydrates into human lymphocytes through swelling-activated channels, Proceedings of the 1st International Forum on Heat Transfer, (2004), 171-172.
- 53) R. Reuss, J. Ludwig, R. Shirakashi, et al., Introduction of saccharide and sugar alcohols into mammalian cells through swelling-activated permeation, Cryo2005 Abstracts (2005), 34.
- 60) R. Shirakashi, T. Yoshida, C. Provin, et al., Measurement of the energy metabolism of HepG2 cells, Journal of Biomechanics Abstract of the 5th World Congress of Boimechanics (2006) 633.
- 61) R.Reuss, H.Zimmermann, et al., Intracellular inositol delivered through swelling-activated channels offers cryoprotection to Langerhans islets, Cryo2006 Abstracts (2006), 68.
- 62) R.Shirakashi, K.Yamauchi, et al., Electrofusion of Jurkat cells and giant unilamelar liposomes loaded with trehalose, Cryo2006 Abstracts (2006), 96.
- 64) R. Shirakashi, K. Yamauchi, et al., How does extracellular saccharide affect lipid membrane and intracellular ice formation, Cryo2007 Abstracts (2007), 39.
- 65) R. Shirakashi, K. Kasahara, et al., Dielectric Spectroscopy and Relaxation Analysis of Methane Hydrate, Proceedings of the 8th Asian Thermophysical properties Conference, (2007), 142.
- 77) R. Shirakashi, K.Taniguchi, M. Kuroda, Mechanism of Producing Giant Unilamellar Vesicles by Electroformation, Proceedings of 2nd International Forum on Heat Transfer, (2008), 89.
- 78) R.Shirakashi, et al., Calculation of Cellular Memebrane potential by FEM Electric Field Analysis, Proceedings of 2nd International Forum on Heat Transfer, (2008), 85.
- 79) K.Takano, C.Provin, et al., Measurement of Flow Characteristics and Oxygen Metabolism of Plate Type Bioreactor, The 7th JSME-KSME Thermal and Fluids Engineering Conference (2008), 183.
- 88) R.Shirakashi, Qiaoqiao Shen, Effects of trehalose on ATP degradative activity of F1-/F0F1-ATPase membrane protein, Cryo2009 Abstracts (2009), 57.
- 96) R. Shirakashi, R. Sakane, J. Yamada, Dielectric spectrum and water activity of swine skin gelatine- gel, 6th BDS Applications; Book of Abstract, (2010), 255.
- 105) K. Furuta, et al., An Attempt to Establish a Network of Bioresource Facilities in Japan, BIOPRESERVATION AND BIOBANKING, Vol.10, No.2 (2012), 6.
- 107) V.L. Sukhorukov, R. Shirakashi, Changes in the dielectric properties of medaka fish embryos during development, BDS2012 (2012) 118.
- 108) R. Shirakashi, V.L. Sukhorukov, Dielectric spectroscopy of medaka fish embryos using Electrorotation, BDS2012 (2012) 134.
- 128) H. Kawashima, R. Shirakashi, M. Uemura, DEVELOPMENT OF NUMERICAL SIMULATION MODEL FOR MICROGROOVE EVAPORATOR, PRTEC, (2016) PRTEC-15052.
- 129) R. Shirakashi, ELECTRO MICROINJECTION FOR LOADING LARGE MOLECULES INTO FISH EGG, PRTEC, (2016) PRTEC-14601.
- 133) R. Shirakashi, Y. Amano, J. Yamada, Temperature Dependent β-relaxation time of ε-Polylysine Aqueous Solution, ATPC2016, (2016).
- 143) Kiyoshi Takano, Ryo Shirakashi, DEVELOPMENT OF DRY-PRESERVATION TECHNOLOGY FOR BIOLOGICAL PROTEIN, 9th Asian Conference on Refrigeration and Air Conditioning (2018).
- 144) Ryo Shirakashi, Sikai Wang, Electric field analysis for electro-piercing of medaka eggs, 15th International Conference on Flow Dynamics (2018), 572-573.
- 145) Dmitri Sisario, Ryo Shirakashi, et al., The Role of Small Organic Osmolytes in the Volume Regulation and Migration of Cancer Cells, 15th ICFD (2018), 592-593.
- 154) Ryo Shirakashi, Masatake Aoki, INIHIBITION OF INTRACELLULAR ICE FORMATION IN EMBRYONIC CELLS OF MEDAKA FISH, PRTEC-24215 (2019).
- 158) Lin Wei and Ryo Shirakashi, Measurement of Diffusion Coefficient and Hydrogen Bond of Water in Dry-protective Solution, CRYO2020 (2020).
- 159) Lin Wei and Ryo Shirakashi, Numerical Simulation of Water Distributions in Bio-protective Solution During Drying Process, 17th ICFD (2020) p.280-281.
- 165) Junkai Zhang, et al., Relationship between Dielectric and Infrared Spectra of Water, 18th ICFD (2021) p.371-373.
- 166) Ryo Shirakashi, Kinetics and Hydrogen-bond States of Water Molecules in Protein Solutions and Biomaterials, 18th ICFD (2021) p.368-370.
- 173) Kang Hu, Ryo Shirakashi, Electric field alignment dominates the water rotation dynamics in protein solutions, ATPC2022 (2022).
- 175) Kang Hu, Ryo Shirakashi, Coupling Between the Rotational and Translational Dynamics of Water in Lysozyme Solutions, 19th ICFD (2022).
- 180) Kang Hu, Ryo Shirakashi, Molecular dynamics studies of sugar solutions for controlling water rotational relaxation time, SB3C2023 (2023) p.275-276.
- 182) Kosei Kawai, Ryo Shirakashi, Measurement of 2D-distribution of water rotational relaxation time by SWIR, 22nd ECTP (2023) p.66.
- 183) Hiroaki Matsuura, Ryo Shirakashi, Exclusion of Ohmic loss effect from dielectric loss spectra, 22nd ECTP (2023) p.189.
- 187) Koh FURUTA, et al., Filter-Biobanking: Possibility in Waste Water Based Epidemiology(WBE), ISBER Regional Symposium (2023).
- 189) K. Furuta, et al., Potential Value of Biobanking in Waste Water, ISBER 2024 Annual Meeting, A-37.
- 190) K. Furuta, et al., Decarbonization in Biobanking, ISBER 2024 Annual Meeting, A-37.
- 192) Hiroaki Matsuura, Ryo Shirakashi, MEASUREMENT OF SLOW DYNAMICS OF INTRACELLULAR WATER BY DIELECTRIC SPECTROSCOPY, 3rd ACTS (2024).
- 196) K. Hu, et al., The Molecular Nature behind the Dielectric Spectra in the Gigahertz Domain, BDS2024, P18-S04.
- 197) K. Kawai, et al., Measurement of rotational relaxation time of water in biosystem by SWIR micro spectroscopy, BDS2024, YRS5-S04.
- 198) H. Matsuura, et al., Slow dynamics of intracellar water measured by BDS with Kramers-Kronig relations, BDS2024, OC29-S04.
- 201) R. Shirakashi, J. Zhang, SHORT WAVE INFRARED SPECTROSCOPY FOR MEASURING INTRACELLULAR WATER ROTATIONAL RELAXATION TIME, PRTEC-24072 (2024).
- 203) K. Kawai and R. Shirakashi, Measurement of water-retained samples by SWIR spectroscopy and its thermodynamic interpretation, 62nd National Heat Transfer Symposium (2025).
- 204) H. Matsuura and R. Shirakashi, Development of dry-preservation technology for liposome drugs, 62nd National Heat Transfer Symposium (2025).
- 207) H. Matsuura and R. Shirakashi, Development of measurement technique of Soret and mass diffusion coefficients, Biothermology Workshop 2025.
4. Research Report
- 32) Shigefumi Nishio and Ryo Shrakashi, Personal Report Professor Tanasawa, International Journal of Heat and Mass Transfer 47(21), (2004), 4505-4506.
- 60) J.W. Rose, S. Nishio, et al., In Memoriam Professor Ichiro Tanasawa (1936-2014), International Journal of Heat and Mass Transfer 85 (2015), 916-917
- 78) Ryo Shirakashi, et al., In memoriam - Professor Shigefumi Nishio (1949-2022), International Journal of Heat and Mass Transfer 226 (2024) 125466.