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1. Original Paper (full paper with peer Review)

  1. 2) Ryo Shirakashi and Ichiro Tanasawa, Numerical Simulation of Freezing Process of Biological Tissues, HEAT TRANSFER-JAPANESE RESEARCH vol.24 No.4.(1995), 342-355.
  2. 8) Minoru Hosoda, Shigefumi Nishio, Ryo Shirakashi, STUDY OF MEANDERING CLOSED-LOOP HEAT-TRANSPORT DEVICE (VAPOR-PLUG PROPAGATION PHENOMENA), JSME International Journal vol.42, No.4 Series B (1999), 737-744.
  3. 9) Ryo Shirakashi and Ichiro Tanasawa, Measurement of Critical Osmotic Pressure for Biological Cells (Minimum Cell-Volume-Ratio as an Estimation of Viability), JSME International Journal vol.42, No.3 Series B (1999), 561-565.
  4. 10) Ryo Shirakashi and Ichiro Tanasawa, The Method of Designing Prefreezing and Freezing Process of Biological Tissues, JSME International Journal vol.42, No.3 Series C (1999), 790-795
  5. 15) Ryo Shirakashi, C.M.Kostner, K.J. Muller, M.Kurschner, U.Zimmermann and V.Sukhorukov, Intracellular Delivery of Trehalose into Mammalian Cells by Electropermeabilization, Journal of Membrane Biology vol.189 (2002), 45-54.
  6. 16) Shigefumi Nishio, Shin-ichi Nagata, Shohei Numata and Ryo Shirakashi, Study on Thermal Characteristics of Bubble-Driven Heat-Transport Device, Heat Transfer - Asian Research, 32 (2), (2003), 167-177.
  7. 18) Ryo Shirakashi, Killian J. Muller, Vladimir L. Sukhorukov and Ulrich Zimmermann, Effects of Physiological Isotonic Cryoprotectants on Living Cells During the Freezing-Thawing Process and Effects of Their Uptake by Electroporation: Sp2 Cells in Alginate - Trehalose Solutions, Heat Transfer - Asian Research, 32 (6), (2003), 511-523.
  8. 20) Ryo Shirakashi, Vladimir L. Sukhorukov, Ichiro Tanasawa and Ulrich Zimmermann, Measurement of the permeability and resealing time constant of the electroporated mammalian cell membranes, International Journal of Heat and Mass Transfer 47(21), (2004), 4517-4524.
  9. 21) Randolph Reuss, Jorg Ludwig, Ryo Shirakashi, Friederike Ehrhart, Heiko Zimmermann, Stephan Schneider, Matthias M. Weber, Ulrich Zimmermann, Heike Schneider, and Vladimir L.Sukhorukov, Intracellular delivery of carbohydrates into mammalian cells through swelling-activated pathways, Journal of Membrane Biology vol.200 (2004), 67-81.
  10. 27) V. L. Sukhorukov J. M. Endter, D. Zimmermann, R. Shirakashi, S. Fehrmann, M. Kiesel, R. Reuss, D. Becker, R. Hedrich, E. Bamberg, Th. Roitsch and U. Zimmermann, Mechanisms of Electrically Mediated Cytosolic Ca2+ Transients in Aequorin-Transformed Tobacco Cells, Biophysical Journal Vol.93 (2007), 3324-3337.
  11. 28) R. Shirakashi, K. Kasahara, S. Miura, K. Tsuyuki and D. Tajima, Dielectric Spectroscopy and Relaxation Analysis of Methane Hydrate, High Temperatures High Pressures, Vol.37, No.1 (2008), 71-80.
  12. 29) C.Provin, K.Takanob, Y.Sakai, T.Fujii, R. Shirakashi, A method for the design of 3D scaffolds for high-density cell attachment and determine of optimum perfusion culture conditions, Journal of Biomechanics, Vol.41, No.7 (2008), 1436-1449.
  13. 34) Ryo Shirakashi, Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov, and Ulrich Zimmermann, Evaluation of FEM Electric Field Analysis Using the Electric Properties of Giant Unilamellar Vesicles (GUV) and Jurkat Cells Measured by Electrorotation Spectroscopy : Introduction of Cryo- and Lyoprotectants by Electrofusion of GUVs and Living Cells, Heat Transfer Asian Research, Vol. 38 (3) (2009), 168-182.
  14. 35) C.Provin, K.Takano, T. Yoshida, Y. Sakai, T. Fujii, R.Shirakashi, Low O2 metabolism of HepG2 cells cultured at high density in a 3D microstructured scaffold, Biomedical Microdevices, Vol.2 Issue 11 (2009), 485-494.
  15. 36) Vladimir L. Sukhorukov, Dennis Imes, Michael W. Woellhaf, Joseph Andronic, Martin Kiesel, Ryo Shirakashi, Ulrich Zimmermann, Heiko Zimmermann, Pore size of swelling-activated channels for organic osmolytes in Jurkat lymphocytes, probed by differential polymer exclusion, Biochemica et Biophysica Acta (BBA) - Biomemebranes, Vol.1788 (2009), 1841-1850
  16. 37) R. Shirakashi, I. Hagiya, S. Nishio, Study on high performance evaporator with micro-grooves (Measurement of capillary force in a single groove and modeling of heat and mass transfer), Heat Transfer - Asian Research, Vol.38(6) (2009), 331-346.
  17. 38) S.Ueno, R. Shirakashi, K. Kudoh, T.Higuchi, G. Do, T.Araki, Y.Sagara, Effect of the grain boundary of ice crystals in a frozen gelatin solution on the dielectric properties at a subzero temperature, Bioscience Biotechnology and Biochemistry, Vol.73(11), (2009), 2478-2482
  18. 41) Ryo SHIRAKASHI, Vladimir L. SUKHORUKOV, Randolph REUSS, Alexander SCHULZ, Ulrich ZIMMERMANN, Effects of a Pulse Electric Field on Electrofusion of Giant Unilamellar Vesicle (GUV)-Jurkat Cell (Measurement of Fusion Ratio and Electric Field Analysis of Pulsed GUV-Jurkat Cell) , Journal of Thermal Science and Technology Vol. 7, No. 4 (2012), 589-602.
  19. 43) Ryo Shirakashi, Miriam Mischke, Peter Fischer, Simon Memmel, Georg Krohne, Gunter R. Fuhr, Heiko Zimmermann, Vladimir L. Sukhorukov, Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation, Biochemical and Biophysical Research Communications, Vol. 428 (2012), 127-131.
  20. 44) Shigenao Maruyama, Koji Deguchi, Masazumi Chisaki, Junnosuke Okajima, Atsuki Komiya, Ryo Shirakashi, Proposal for a low CO2 emission power generation system utilizing oceanic methane hydrate, Energy 47 (2012) 340-347.
  21. 45) Ryo Shirakashi, Tsuyoshi Ogawa, Jun Yamada, Dielectric spectroscopy of thin surface layer by Differential Time Domain Reflectometry using a Coplanar Waveguide Circuit line probe, Measurement Science and Technology, Vol. 24, No.2 (2013) 025501+10.
  22. 47) Yanxin Hu, Jiang Cheng, Wei Zhang, Ryo Shirakashi, Shuangfeng Wang, Thermal performance enhancement of grooved heat pipes with inner surface treatment, International Journal of Heat and Mass Transfer, Vol. 67 (2013) 416-419.
  23. 48) Zirong Lin, Shuangfeng Wang, Ryo Shirakashi, L. Winston Zhang, Simulation of a miniature oscillating heat pipe in bottom heating mode using CFD with unsteady modeling, International Journal of Heat and Mass Transfer, Vol.57 (2013) 642-656.
  24. 50) Joseph Andronic, Ryo Shirakashi, Simone U. Pickel, Katherine M. Westerling, Teresa Klein, Thorge Holm, Markus Sauer, Vladimir L. Sukhorukov, Hypotonic activation of the myo-inositol transporter SLC5A3 in HEK293 cells probed by cell volumetry, confocal and super-resolution microscopy, PLOS ONE, DOI:10.1371, journal.pone.0119990 (2015)
  25. 51) Ryo Shirakashi, Tatsuo Yasui,1 Simon Memmel, Vladimir L. Sukhorukov, Electro-microinjection of fish eggs with an immobile capillary electrode, BIOMICROFLUIDICS 9, 064109 (2015)
  26. 52) Takao Furuki, Takahiro Watanabe, Tadaomi Furuta, Kiyoshi Takano, Ryo Shirakashi, Minoru Sakurai, The dry preservation of giant vesicles using a Group 3 LEA protein model peptide and its molecular mechanism, Bulletin of the Chemical Society of Japan, 89 (2016) 1493
  27. 53) Ryo Shirakashi, Yuki Amano, Jun Yamada, Measurement of the Water Relaxation Time of ε-Polylysine Aqueous Solutions, International Journal Thermophysics, DOI 10.1007/s10765-017-2213-y (2017) 38:75
  28. 54) Yukiko Otsuka, Ryo Shirakashi, Kazuhiko Hirakawa, Bound states of water in gelatin discriminated by near-infrared spectroscopy, Japanese Journal of Applied Physics 56, 111602 (2017) doi.org/10.7567/JJAP.56.111602
  29. 55) Ryo Shirakashi, Kiyoshi Takano, Recrystallization and Water Absorption Properties of Vitrified Trehalose Near Room Temperature, Pharmaceutical Research 35: 139 (2018) https://doi.org/10.1007/s11095-018-2420-7
  30. 56) Kiyoshi Takano, Ryo Shirakashi, Development of Dry-preservation technology for biological protein at room temperature - measurement of glass transition temperature and residual activity of lactate dehydrogenase -, Transaction of the Japan Society of Refregerating and Air Conditioning Engineers Vol.35, No.4, 325-330 (2018)
  31. 57) Yuan Pang, Stephanie Sutoko, Yohei Horimoto, Ding Weng, Kevin Montagne, Kikuo Komori, Kiyoshi Takano, Ryo Shirakashi, Masahiro Anzai, Toshiki Niino,Yasuyuki Sakai, Biodegradable and hollowed micro-scaffolds for improved modularassembly-based tissue engineering: Design, 3D fabrication, and feasibility inrandomly packed perfusion culture, Biochemical Engineering Journal, Vol. 149, 15 (2019) 107239
  32. 58) Sikai Wang, Ryo Shirakashi, Local Joule heating and electric force on biological membrane duringelectro-microinjection, International Journal of Heat and Mass Transfer 140 (2019) 798-806
  33. 59) Lin Wei, Ryo Shirakashi, Measurement of the diffusion coefficient and hydrogen bonds of water in a dry-protective solution by microscopic near-infrared spectroscopy, Food and Bioproducts Processing, vol. 124, 97?106 (2020)
  34. 60) Lin Wei, Ryo Shirakashi, Effect of Relaxation Times in a Preservative Solution on Protein Deterioration Rate, Journal of physical Chemistry B, 124, 8741-8749(2020)
  35. 61) Lin Wei, Ryo Shirakashi, Simulation of air/vacuum desiccation process for high-quality preservation of proteins, Journal of Food Process Engineering, DOI: 10.1111/jfpe.13962 (2022)
  36. 62) Hiroaki Matsuura and Ryo Shirakashi, Exclusion of DC conductivity effect from dielectric loss spectrum using Kramers?Kronig relations for evaluation of slow dynamics of water molecules, Japanese Journal of Applied Physics 61, 068003 (2022)
  37. 63) Junkai Zhang, Hiroaki Matsuura, Ryo Shirakashi, Prediction of Water Relaxation Time Using Near Infrared Spectroscopy, Journal of Food Process Engineering, e14095, https://doi.org/10.1111/jfpe.14095 (2022)
  38. 64) Kang Hu, Hiroaki Matsuura, Ryo Shirakashi, Stochastic Analysis of Molecular Dynamics Reveals the Rotation Dynamics Distribution of Water around Lysozyme, The Journal of Physical Chemistry PartB SectionB, 126, 24, 4520?4530 (2022) https://doi.org/10.1021/acs.jpcb.2c00970
  39. 65) Ryo Shirakashi, Zisis Kozlakidis, Birendra Kumar Yadav, Wayne Ng, Jajah Fachiroh, Hanh Vu, Tatsuaki Tsuruyama, Koh Furuta, Decarbonization in Biobanking: A Potential New Scientific Area, BIOPRESERVATION AND BIOBANKING 20, 5, (2022) DOI: 10.1089/bio.2022.0146
  40. 66) Ryo Shirakashi, Dmitri Sisario, Danush Taban, Tessa Korsa, Sophia B. Wanner, Julia Neubauer, Cholpon S. Djuzenova, Heiko Zimmermann, Vladimir L. Sukhorukov, Contraction of the rigor actomyosin complex drives bulk hemoglobin expulsion from hemolyzing erythrocytes, Biomechanics and Modeling in Mechanobiology, (2022). https://doi.org/10.1007/s10237-022-01654-6
  41. 67) Philippa K Brosch, Tessa Korsa, Danush Taban, Patrick Eiring, Sascha Hildebrand, Julia Neubauer, Heiko Zimmermann, Markus Sauer, Ryo Shirakashi, Cholpon S Djuzenova, Dmitri Sisario, Vladimir L Sukhorukov, Glucose- and inositol-transporters, SLC5A1 and SLC5A3, in glioblastoma cell migration, Cancers, 14, 5794 (2022). https://doi.org/10.3390/cancers14235794
  42. 68) Kang Hu, Ryo Shirakashi, Dynamic Electric Field Alignment Determines the Water Rotational Motion around Protein, The Journal of Physical Chemistry PartB SectionB, 127, 6, 1376?1384 (2023), https://doi.org/10.1021/acs.jpcb.2c07405 (Feb)
  43. 69) Kang Hu, Ryo Shirakashi, Molecular dynamics study of water rotational relaxation in saccharide solution for the development of bioprotective agent, Journal of Molecular Liquids, 380,121707 (2023), https://doi.org/10.1016/j.molliq.2023.121707 ?(magagine is June, Version of Record 29 March 2023)
  44. 70) Junkai Zhang, Hiroaki Matsuura, Ryo Shirakashi, A method for measuring dielectric relaxation of water by NIR spectroscopy: Applicability and application to measurement of water diffusion coefficient, Journal of Food Process Engineering, e14335, https://doi.org/10.1111/jfpe.14335 (April)
  45. 71) Hiroaki Matsuura, Kiyoshi Takano, Ryo Shirakashi, Slow water dynamics in dehydrated human Jurkat T cells evaluated by dielectric spectroscopy with Bruggeman?Hanai equation, RSC Advances, 13 (2023), 20934?20940, DOI: 10.1039/d3ra02892e
  46. 72) Junkai Zhang, Ryo Shirakashi, Measurement of the rotational relaxation time of intracellular water in dried yeast and Jurkat cells by near infrared spectroscopy, Biochemical and Biophysical Research Communications, Vol. 710 (2024), 149857, https://doi.org/10.1016/j.bbrc.2024.149857
  47. 73) Kang Hu, Ryo Shirakashi, Determination of the first hydration layer from the perspective of local electric field reorientation, Chemical Physics Letters, 845, 16 (2024), 141302, https://doi.org/10.1016/j.cplett.2024.141302
  48. 75) Kosei Kawai, Ryo Shirakashi, Water rotational relaxation time measurement by shortwave infrared micro spectroscopy(SWIR) at sub-zero temperatures, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 322 (2024) 124707, https://doi.org/10.1016/j.saa.2024.124707
  49. 76) Yuanheng Zhao, Hiroaki Matsuura, Ryo Shirakashi, Water rotational relaxation time of preservation solutions relates to storage lifespan of cells in isochoric cryopreservation system, Food and Bioproducts Processing 147 (2024) 199?208, https://doi.org/10.1016/j.fbp.2024.06.012
  50. 77) Masaaki Kitajima,1 Hirohisa Abe, Ryo Honda, Hiroyuki Kobayashi, Tomohiro Kuroita, Ayuko Nemoto, Ryo Shirakashi, Rodney Scott and Koh Furuta, Biobanking: Possibilities for Wastewater-Based Epidemiology, Biopreservation and Biobanking, Vol.23 (2025) https://doi.org/10.1089/bio.2024.0118
  51. 78) Hanh Vu, Daniel Adamek, Armin Ahmadi, Jerome Baudry, Jajah Fachiroh, Neil Fleshner, Gregory H. Grossman, Paul Hofman, Marius Ilie, Zisis Kozlakidis, Birendra Kumar Yadav, Elodie Long-Mira, Vineetha Menon, Wayne Ng, Lee Organick, Swee Heng Milon Pang, Naghmeh Rastegar, Ryo Shirakashi, Tiiu Sildva, Heidi Wagner, Koh Furuta, Experts Speak Forum: Decarbonization for Green Biobanking?The Current Landscape and Challenges for the Future, Biopreservation and Biobanking, Vol.23 (2025) https://doi.org/10.1089/bio.2024.0128

2. International conference in full papers (full paper with peer Review)

  1. 1) Ryo Shirakashi and Ichiro Tanasawa, Measurement of diffusion coefficient of cryoprotectant mixture in biological tissues, 3rd KSME/JSME Thermal Engineering conference (1996), 267-272.
  2. 2) Ryo Shirakashi and Ichiro Tanasawa, Method of Designing Pre-freezing Protocol in Cryopreservation of Biological Materials, THE ANNALS OF NEW YORK ACADEMY OF SCIENCES, Biotransport: Heat and Mass Transfer in Living Systems, vol.858, (1998), 175-182.
  3. 3) Minoru Hosoda, Shigefumi Nishio, Ryo Shirakashi, MEANDERING CLOSED-LOOP HEAT-TRANSPORT TUBE (PROPAGATION PHENOMENA OF VAPOR PLUG), 5th ASME /JSME Joint Thermal Engineering Conference AJTE99-6306 (1999), (CD-ROM only).
  4. 4) Xianglan BAI, Ryo SHIRAKASHI and Shigefumi NISHIO, ON THE MECHANISM OF AGING EFFECTS ON THE DIELECTRIC PROPERTIES OF POLYCRYSTALLINE ICE, Proceedings of the 4th JSME-KSME Thermal Engineering Conference, vol.3, (2000), 325-330.
  5. 5) Tetsuya Araki, Ryo Shirakashi and Yasuyuki Sagara, Numerical Simulation of Freezing Process of Coffee Solution Related to Freeze-Drying, Proceedings of 2nd Asian-Oceania Drying Conference, (2001), 155-163.
  6. 6) S.Nishio, S.Nagata, S.Baba and R.Shirakashi, Thermal performance of SEMOS heat pipes, 12th International Heat Transfer Conference vol.4 (2002), 477-482.
  7. 7) R.Shirakashi, BAI Xianglan and S.Nishio, Dielectric Property of Ice for Rapid and Uniform Dielectric Heating- for thawing ice crystals in biological materials -, The 6th ASME-JSME Thermal Engineering Joint Conference -AJTC 2003- TED-AJ03-274 (2003),(CD-ROM only).
  8. 8) K.KAMINISHI, T.ARAKI, R.SHIRAKASHI, Y.SAGARA, THREE-PHASE MODEL FOR SIMULATING FREEZING PROCESS OF AQUEOUS SOLUTIONS, Inter National Congress of Refrigeration ICR0232 (2003), (CD-ROM only).
  9. 9) R.Shirakashi, The long term preservation of living biomaterials - Introduction of cryo/lyoprotective agents into cells -, Proceedings of the 3rd Korea-Japan Joint Seminar on Heat and Mass Transfer: Challenging Researches in Thermal Engineering (2005), 17-25.
  10. 10) C. Provin, K. Takano, R. Shirakashi, Y. Sakai, T. Fujii, Fabrication of Microbioreactors with an Optimized Structure Designed for High Density Culture of Hepatocyte, 2006 Inernatinal Conference on Microtechnologies in Medicine and Biology IEEE Cat.No. 06EX1357C (2006), 267-270. (CD-ROM only).
  11. 11) Ryo Shirakashi, Tomomi Yoshida, Christophe Provin, Kiyoshi Takano,Yasuyuki Sakai, Teruo Fujii, Steady measurement of glucose metabolism of hepatocyte, ASME-JSME Thermal Engineering and Summer Heat Transfer Conference, HT2007-32750 (2007), (6Pages CD-ROM only).
  12. 12) Isao HAGIYA, Shigefumi NISHIO, Ryo SHIRAKASHI, Heat and Mass Transfer Characteristics of Single Micro Groove for Micro Groove Evaporator, The 3rd International Conference on cooling and Heating Technology (2007) 34-38.
  13. 13) M. Maruyama, M. Chisaki, A. Komiya and R.Shirakashi, A proposal of low CO2 emission power generation system utilizing oceanic methane hydrate, Proceedings of the International Conference on Power Engineering-09, (2009) 2-343-2-348.
  14. 14) Ryo Shirakashi, Kiyoshi Takano, Christophe Provin, Yasuyuki Sakai, Teruo Fujii, Measurement of Cellular Adhesion Ratios under High Shear Flow on Various Substrates, Proceedings of the 8th ASME-JSME Thermal Engineering Joint Conference, (2011), (CD-ROM) AJTEC2011-44404.

3. Conference Abstracts

  1. 1) Ryo Shirakashi and Ichiro Tanasawa, Numerical Simulation of Freezing Process of Biological Tissues, The 31st Annual Meeting of The Society for Cryobiology (1994) (Cryobiology, Vol.31, No.6 (1994), 604-605.)
  2. 13) Ryo Shirakashi and Ichiro Tanasawa,MEASUREMENT OF MEMBRANE PERMEABILITY OF ENDOTHELIAL CELL, Third World Congress of Biomechanics ABSTRACTS, (1998), 181.
  3. 25) Ryo Shirakashi and Yasuyuki Sakai, Enhancement of membrane permeability by electroporation, The 37st Annual Meeting of The Society for Cryobiology in Association with The Society for Low Temperature Biology, Cryobiology 2000 (2000) (Cryobiology, Vol.41, No.4 (2000),356.)
  4. 26) Ryo Shirakashi, Xianglang Bai and Shigefumi Nishio, Measurement of dielectric-loss spectrum of ice for rapid-uniform thawing, The 37st Annual Meeting of The Society for Cryobiology in Association with The Society for Low Temperature Biology, Cryobiology 2000 (2000) (Cryobiology, Vol.41, No.4 (2000), 368.)
  5. 30) Tetsuya Araki, Ryo Shirakashi and Yasuyuki Sagara, A Numerical Model for Freezing of Coffee Solutions as Preliminary Operation of Freeze-drying Process, Proceedings of 7th Conference of Food Engineering - AIChE Annual Meeting, (2001)(No trabel for NY terrorist Attack).
  6. 40) Shigefumi Nishio, Takeshi Iwakami, Ryo Shirkashi and Makoto Mita, Study of Micro-Miniture JT Cooler, Thermal Science and Engineering, vol.11, No.4 -Special issue for extended abstracts of papers presented at NHTSJ (2003), 29-30.
  7. 41) Ryo Shirakashi, Kilian J. Muller, Vladimir L. Sukhorukov, Ichiro Tanasawa and Ulrich Zimmermann, Effects of Saccharide on Living Cells during the Freezing-Thawing Process and Effects of their uptake by Electroporation, Cryobiomol 2003, Annual Meeting of The Society for Cryobiology in Association with The Society for Low Temperature Biology (2003) (Cryobiology, Vol.47 , No.3 (2003), 283.)
  8. 49) Ryo Shirakashi, Effect of saccharide on intracellular ice formation, cryomedicine2004 Main Program and Abstracts, (2004), 28. (Low Temperature Medicine, Vol.30, No.3 (2004), 80.)
  9. 50) Randolph Reuss, Ryo Shirakashi, Friederike Ehrhart, Heiko Zimmermann, Ulrich Zimmermann, Heike Schneider, and Vladimir L.Sukhorukov, Introduction of carbohydrates into human lymphocytes through swelling-activated channels, Proceedings of the 1st International Forum on Heat Transfer, (2004), 171-172.
  10. 53) R. Reuss, J. Ludwig, R. Shirakashi, F. Ehrhart, H. Zimmermann, S. Schneider, M. M. Weber, U. Zimmermann, H. Schneider, V. L. Sukhorukov, Introduction of saccharide and sugar alcohols into mammalian cells through swelling-activated permeation, Cryo2005 Abstracts (2005), 34. (Cryobiology, Vol.51, No.3 (2005), 401.)
  11. 60) R. Shirakashi, T. Yoshida, C. Provin, K. Takano, Y. Sakai, T. Fujii, Measurement of the energy metabolism of HepG2 cells for designing engineering tissues allowing high density cultivation, Journal of Biomechanics Abstract of the 5th World Congress of Boimechanics,Vol.39, Supplyment 1 (2006) 633.
  12. 61) R.Reuss, H.Zimmermann, F.Ehrhart, P.J.Feilen, M.M.Weber, R.Shirakashi, U.Zimmermann, V.L.Sukhorukov, Intracellular inositol delivered through swelling-activated channels offers cryoprotection to Langerhans islets, Cryo2006 Abstracts (2006), 68. (Cryobiology, Vol. 53, No.3 (2006), 412-413.)
  13. 62) R.Shirakashi, K.Yamauchi, R.Reuss, U.Zimmermann, V.L.Sukhorukov, Electrofusion of Jurkat cells and giant unilamelar liposomes loaded with trehalose, Cryo2006 Abstracts (2006), 96. (Cryobiology, Vol. 53, No.3 (2006), 397)
  14. 64) R. Shirakashi, K. Yamauchi, M. Kuroda, M. Nagasawa, S. Fujikawa, How does extracellular saccharide affect lipid membrane and intracellular ice formation-, Cryo2007 Abstracts (2007), 39 (Cryobiology, Vol.55, No.3 (2007), 334.)
  15. 65) R. Shirakashi, K. Kasahara, S. Miura, K. Tsuyuki and D. Tajima, Dielectric Spectroscopy and Relaxation Analysis of Methane Hydrate, Proceedings of the 8th Asian Thermophysical properties Conference, (2007), 142.
  16. 77) R. Shirakashi, K.Taniguchi, M. Kuroda, Mechanism of Producing Giant Unilamellar Vesicles by Electroformation, Proceedings of 2nd International Forum on Heat Transfer, (2008), 89. (CD-ROM, No.212)
  17. 78) R.Shirakashi, Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov, Ulrich Zimmermann, Calculation of Cellular Memebrane potential by FEM Electric Field Analysis using Cellular Electric Properties from Electrorotation Spectroscopy, Proceedings of 2nd International Forum on Heat Transfer, (2008), 85. (CD-ROM, No.148)
  18. 79) K.Takano, C.Provin, Y.Sakai, T.Fujii and R.Shirakashi, Measurement of Flow Characteristics and Oxygen Metabolism of Plate Type Bioreactor for in vitro High-density Cell Culture, The 7th JSME-KSME Thermal and Fluids Engineering Conference Book of Abstracts, (2008), 183. (CD-ROM, No.08-201: H313)
  19. 88) R.Shirakashi, Qiaoqiao Shen, Effects of trehalose on ATP degradative activity of F1-/F0F1-ATPase membrane protein, Cryo2009 Abstracts (2009), 57. (Cryobiology, Vol. 59, No.3 (2009), 394.)
  20. 96) R. Shirakashi, R. Sakane, J. Yamada, Dielectric spectrum and water activity of swine skin gelatine- gel, 6th International conference on Broadband Dielectric Spectroscopy and its Applications; Book of Abstract, (2010), 255.
  21. 105) K. Furuta, K. Matsushita, Y. Goto, Y. Miyagi, M. Sawabe, R. Shirakashi, T. Takeuchi, T. Masui, I. Aoki, A. Nakagawara, An Attempt to Establish a Network of Bioresource Facilities in Japan, BIOPRESERVATION AND BIOBANKING, Vol.10, No.2 (2012), 6.
  22. 107) V.L. Sukhorukov, R. Shirakashi, Changes in the dielectric properties of medaka fish embryos during development studied by electrorotation, Proceedings of Broadband Dielectric Spectroscopy and its Applications (BDS2012) (2012) 118.
  23. 108) R. Shirakashi, V.L. Sukhorukov, Dielectric spectroscopy of medaka fish embryos using Electrorotation, Proceedings of Broadband Dielectric Spectroscopy and its Applications (BDS2012) (2012) 134.
  24. 128) H. Kawashima, R. Shirakashi, M. Uemura, DEVELOPMENT OF NUMERICAL SIMULATION MODEL FOR PREDICTING HEAT AND MASS TRANSFER IN MICROGROOVE AND FOR DESIGNING HIGH PERFORMANCE MICROGROOVE EVAPORATOR, Proceedings of the First Pacific Rim Thermal Engineering Conference, PRTEC, (2016) PRTEC-15052 (web only)
  25. 129) R. Shirakashi, ELECTRO MICROINJECTION FOR LOADING LARGE MOLECULES INTO FISH EGG, Proceedings of the First Pacific Rim Thermal Engineering Conference, PRTEC, (2016) PRTEC-14601 (web only)
  26. 133) R. Shirakashi, Y. Amano, J. Yamada, Temperature Dependent β-relaxation time of ε-Polylysine Aqueous Solution, Proceedings of 11th Asian Thermophysical Properties Conference, ATPC2016 (web only), (2016), OS10 Invited
  27. 143) Kiyoshi Takano, Ryo Shirakashi, DEVELOPMENT OF DRY-PRESERVATION TECHNOLOGY FOR BIOLOGICAL PROTEIN AT ROOM TEMPERATURE, Proceedings of the 9th Asian Conference on Refrigeration and Air Conditioning, (USB) (2018), OS-15-1, ISBN 978-4-88967-138-4
  28. 144) Ryo Shirakashi, Sikai Wang, Electric field analysis for electro-piercing of medaka eggs by frequency domain finite element method, Proceedings of 15th International Conference on Flow Dynamics, (USB) (2018), 572-573.
  29. 145) Dmitri Sisario, Ryo Shirakashi, Vladimir, Sukhorukov, The Role of Small Organic Osmolytes in the Volume Regulation and Migration of Cancer Cells, Proceedings of 15th International Conference on Flow Dynamics, (USB) (2018),592-593.
  30. 154) Ryo Shirakashi, Masatake Aoki, INIHIBITION OF INTRACELLULAR ICE FORMATION IN EMBRYONIC CELLS OF MEDAKA FISH, Extended Abstracts of The Second Pacific Rim Thermal Engineering Conference, (Web) (2019), PRTEC-24215.
  31. 158) Lin Wei and Ryo Shirakashi, Measurement of Diffusion Coefficient and Hydrogen Bond of Water in Dry-protective Solution by Microscopic NIR Spectroscopy, Proceedings of CRYO2020, (web?) (2020)
  32. 159) Lin Wei and Ryo Shirakashi, Numerical Simulation of Water Distributions in Bio-protective Solution During Drying Process, Proceedings of 7th International Conference on Fluid Dynamics, (2020) p.280-281.
  33. 165) Junkai Zhang, Hiroaki Matsuura, Ryo Shirakashi, Relationship between Dielectric and Infrared Spectra of Water:Hydrogen Bond Strength and Rotational Relaxation Time in Saccharide Aqueous Solutions, Proceedings of 18th International Conference on Fluid Dynamics, (2021) p.371-373.
  34. 166) Ryo Shirakashi, Kinetics and Hydrogen-bond States of Water Molecules in Protein Solutions and Biomaterials, Proceedings of 18th International Conference on Fluid Dynamics, (2021) p.368-370.
  35. 173) Kang Hu, Ryo Shirakashi Electric field alignment dominates the water rotation dynamics in protein solutions the 13th Asian Thermophysical Properties Conference, ATPC2022 September 26-30, 2022, Onlin
  36. 175) Kang Hu, Ryo Shirakashi Coupling Between the Rotational and Translational Dynamics of Water in Lysozyme Solutions Nineteenth International Conference on Flow Dynamics November 9 - 11, 2022, Sendai, Miyagi, OS8-4
  37. 180) Kang Hu, Ryo Shirakashi, Molecular dynamics studies of sugar solutions for controlling water rotational relaxation time, SB3C2023 Summer Biomechanics, Bioengineering and Biotransport Conference Proceedings Book (2023) p.275-276.
  38. 182) Kosei Kawai, Ryo Shirakashi, Measurement of 2D-distribution of water rotational relaxation time by short-wave infrared (SWIR) micro spectroscopy, 22nd EUROPEAN CONFERENCE ON THERMOPHYSICAL PROPERTIES Book of Abstracts (2023) p.66
  39. 183) Hiroaki Matsuura, Ryo Shirakashi, Exclusion of Ohmic loss effect from dielectric loss spectra using Kramers-Kronig relations for evaluation of slow dynamics of water molecules, 22nd EUROPEAN CONFERENCE ON THERMOPHYSICAL PROPERTIES Book of Abstracts (2023) p.189
  40. 187) Koh FURUTA, Hirohisa ABE, Ryo HONDA, Masaaki KITAJIMA, Hiroyuki KOBAYASHI, Tomohiro KUROITA, Ayuko NEMOTO, Ryo SHIRAKASHI, Filter-Biobanking:Possibility in Waste Water Based Epidemiology(WBE), ISBER Regional Symposium, Program, (2023) GRANADA, P22-23
  41. 189) K. Furuta, H. Abe, R. Honda, M. Kitajima, H. Kobayashi, T. Kuroita, A. Nemoto, R. Shirakashi, Potential Value of Biobanking in Waste Water, The International Society for Biological and Environmental Repositories Presents Abstracts from Its 2024 Annual Meeting, BIOPRESERVATION AND BIOBANKING Vol.00, No.00, PH-04(2024), A-37, DOI: 10.1089/bio.2024.29133.abstracts
  42. 190) K. Furuta, H. Vu, L. Organick, M. Scott, R. Shirakashi, Z. Kozlakidis, Decarbonization in Biobanking, The International Society for Biological and Environmental Repositories Presents Abstracts from Its 2024 Annual Meeting, BIOPRESERVATION AND BIOBANKING Vol.00, No.00, PH-05 (2024), A-37, DOI: 10.1089/bio.2024.29133.abstracts
  43. 192) Hiroaki Matsuura, Ryo Shirakashi, MEASUREMENT OF SLOW DYNAMICS OF INTRACELLULAR WATER BY DIELECTRIC SPECTROSCOPY, Extended Abstracts of the Third Asian Conference on Thermal Sciences, 3rd ACTS, June 23-27, 2024, Shanghai, China
  44. 196) K. Hu, H. Matsuura, J. Zhang and R. Shirakashi, The Molecular Nature behind the Dielectric Spectra in the Gigahertz Domain, 12th Conference on Broadband Dielectric Spectroscopy and its Applications BDS2024 Book of Abstracts (PDF), P18-S04 (2024)
  45. 197) K. Kawai, J. Zhang, H. Matsuura, R. Shirakashi, Measurement of rotational relaxation time of water in biosystem by shortwave infrared micro spectroscopy, 12th Conference on Broadband Dielectric Spectroscopy and its Applications BDS2024 Book of Abstracts (PDF), YRS5-S04 (2024)
  46. 198) H. Matsuura, K. Kawai and R. Shirakashi, Slow dynamics of intracellar water measured by BDS with Kramers-Kronig relations and Bruggeman?Hanai equation, 12th Conference on Broadband Dielectric Spectroscopy and its Applications BDS2024 Book of Abstracts (PDF), OC29-S04 (2024)
  47. 201) R. Shirakashi, J. Zhang, SHORT WAVE INFRARED SPECTROSCOPY FOR MEASURING INTRACELLULAR WATER ROTATIONAL RELAXATION TIME DURING DESICCATION, Extended Abstracts of The Third Pacific Rim Thermal Engineering Conference, PRTEC-24072 (2024)
  48. 203) K. Kawai and R. Shirakashi, Measurement of water-retained samples by shortwave infrared (SWIR) spectroscopy and its thermodynamic interpretation, The 62nd National Heat Transfer Symposium & HTSJ International Heat Transfer Symposium Book of Abstracts (pdf), IOS8-2-04 (2025)
  49. 204) H. Matsuura and R. Shirakashi, Development of dry-preservation technology for liposome drugs: Preservation of liposomes containing lacate dehydrogenase at room temperature, The 62nd National Heat Transfer Symposium & HTSJ International Heat Transfer Symposium Book of Abstracts (pdf), IOS8-1-05 (2025)
  50. 207) H. Matsuura and R. Shirakashi, Development of measurement technique of Soret and mass diffusion coefficients for aqueous systems by Soret Forced Rayleigh scattering, Biothermology Workshop 2025 Program & Abstract Book(PDF), Poster P.3-P.4 (2025)

4. Research Report

  1. 32) Shigefumi Nishio and Ryo Shrakashi, Personal Report Professor Tanasawa, International Journal of Heat and Mass Transfer 47(21), (2004), 4505-4506.
  2. 60) J.W. Rose, S. Nishio, R. Shirakashi and Y. Utaka, In Memoriam Professor Ichiro Tanasawa (1936-2014), International Journal of Heat and Mass Transfer 85 (2015), 916?917
  3. 78) Ryo Shirakashi, Niro Nagai, Hiroyasu Ohtake, Masahiro Shoji, Vijay K. Dhir, In memoriam - Professor Shigefumi Nishio (1949-2022), International Journal of Heat and Mass Transfer 226 (2024) 125466, https://doi.org/10.1016/j.ijheatmasstransfer.2024.125466

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