１． Original Paper (full paper with peer Review）
2) Ryo Shirakashi and Ichiro Tanasawa, Numerical Simulation of Freezing Process of Biological Tissues, HEAT TRANSFER-JAPANESE RESEARCH vol.24 No.4.(1995), 342-355.
8) Minoru Hosoda, Shigefumi Nishio, Ryo Shirakashi, STUDY OF MEANDERING CLOSED-LOOP HEAT-TRANSPORT DEVICE (VAPOR-PLUG PROPAGATION PHENOMENA), JSME International Journal vol.42, No.4 Series B (1999), 737-744.
9) Ryo Shirakashi and Ichiro Tanasawa, Measurement of Critical Osmotic Pressure for Biological Cells (Minimum Cell-Volume-Ratio as an Estimation of Viability), JSME International Journal vol.42, No.3 Series B (1999), 561-565.
10) Ryo Shirakashi and Ichiro Tanasawa, The Method of Designing Prefreezing and Freezing Process of Biological Tissues, JSME International Journal vol.42, No.3 Series C (1999), 790-795
15) Ryo Shirakashi, C.M.Kostner, K.J. Muller, M.Kurschner, U.Zimmermann and V.Sukhorukov, Intracellular Delivery of Trehalose into Mammalian Cells by Electropermeabilization, Journal of Membrane Biology vol.189 (2002), 45-54.
16) Shigefumi Nishio, Shin-ichi Nagata, Shohei Numata and Ryo Shirakashi, Study on Thermal Characteristics of Bubble-Driven Heat-Transport Device, Heat Transfer - Asian Research, 32 (2), (2003), 167-177.
18) Ryo Shirakashi, Killian J. Muller, Vladimir L. Sukhorukov and Ulrich Zimmermann, Effects of Physiological Isotonic Cryoprotectants on Living Cells During the Freezing-Thawing Process and Effects of Their Uptake by Electroporation: Sp2 Cells in Alginate - Trehalose Solutions, Heat Transfer - Asian Research, 32 (6), (2003), 511-523.
20) Ryo Shirakashi, Vladimir L. Sukhorukov, Ichiro Tanasawa and Ulrich Zimmermann, Measurement of the permeability and resealing time constant of the electroporated mammalian cell membranes, International Journal of Heat and Mass Transfer 47(21), (2004), 4517-4524.
21) Randolph Reuss, Jorg Ludwig, Ryo Shirakashi, Friederike Ehrhart, Heiko Zimmermann, Stephan Schneider, Matthias M. Weber, Ulrich Zimmermann, Heike Schneider, and Vladimir L.Sukhorukov, Intracellular delivery of carbohydrates into mammalian cells through swelling-activated pathways, Journal of Membrane Biology vol.200 (2004), 67-81.
24) M. Kiesel, R. Reuss, J. Endter, D. Zimmermann, H. Zimmermann, R. Shirakashi, E. Bamberg, U. Zimmermann, and V.L. Sukhorukov, Swelling-Activated Pathways in Human T-Lymphocytes Studied by Cell Volumetry and Electorotation, Biophysical Journal vol.90, (2006), 4720-4729.
27) V. L. Sukhorukov J. M. Endter, D. Zimmermann, R. Shirakashi, S. Fehrmann, M. Kiesel, R. Reuss,
D. Becker, R. Hedrich, E. Bamberg, Th. Roitsch and U. Zimmermann, Mechanisms of Electrically Mediated Cytosolic Ca2+ Transients in Aequorin-Transformed Tobacco Cells, Biophysical Journal Vol.93 (2007), 3324-3337.
28) R. Shirakashi, K. Kasahara, S. Miura, K. Tsuyuki and D. Tajima, Dielectric Spectroscopy and Relaxation Analysis of Methane Hydrate, High Temperatures High Pressures, Vol.37, No.1 (2008), 71-80.
29) C.Provin, K.Takanob, Y.Sakai, T.Fujii, R. Shirakashi, A method for the design of 3D scaffolds for high-density cell attachment and determine of optimum perfusion culture conditions, Journal of Biomechanics, Vol.41, No.7 (2008), 1436-1449.
34) Ryo Shirakashi, Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov, and Ulrich Zimmermann, Evaluation of FEM Electric Field Analysis Using the Electric Properties of Giant Unilamellar Vesicles (GUV) and Jurkat Cells Measured by Electrorotation Spectroscopy : Introduction of Cryo- and Lyoprotectants by Electrofusion of GUVs and Living Cells, Heat Transfer Asian Research, Vol. 38 (3) (2009), 168-182.
35) C.Provin, K.Takano, T. Yoshida, Y. Sakai, T. Fujii, R.Shirakashi, Low O2 metabolism of HepG2 cells cultured at high density in a 3D microstructured scaffold, Biomedical Microdevices, Vol.2 Issue 11 (2009), 485-494.
36) Vladimir L. Sukhorukov, Dennis Imes, Michael W. Woellhaf, Joseph Andronic, Martin Kiesel, Ryo Shirakashi, Ulrich Zimmermann, Heiko Zimmermann, Pore size of swelling-activated channels for organic osmolytes in Jurkat lymphocytes, probed by differential polymer exclusion, Biochemica et Biophysica Acta (BBA) - Biomemebranes, Vol.1788 (2009), 1841-1850
37) R. Shirakashi, I. Hagiya, S. Nishio, Study on high performance evaporator with micro-grooves (Measurement of capillary force in a single groove and modeling of heat and mass transfer), Heat Transfer - Asian Research, Vol.38(6) (2009), 331-346.
38) S.Ueno, R. Shirakashi, K. Kudoh, T.Higuchi, G. Do, T.Araki, Y.Sagara, Effect of the grain boundary of ice crystals in a frozen gelatin solution on the dielectric properties at a subzero temperature, Bioscience Biotechnology and Biochemistry, Vol.73(11), (2009), 2478-2482
41) Ryo SHIRAKASHI, Vladimir L. SUKHORUKOV, Randolph REUSS, Alexander SCHULZ, Ulrich ZIMMERMANN, Effects of a Pulse Electric Field on Electrofusion of Giant Unilamellar Vesicle (GUV)-Jurkat Cell (Measurement of Fusion Ratio and Electric Field Analysis of Pulsed GUV-Jurkat Cell) , Journal of Thermal Science and Technology Vol. 7, No. 4 (2012), 589-602.
43) Ryo Shirakashi, Miriam Mischke, Peter Fischer, Simon Memmel, Georg Krohne, Gunter R. Fuhr, Heiko Zimmermann，Vladimir L. Sukhorukov, Changes in the dielectric properties of medaka fish embryos during development, studied by electrorotation, Biochemical and Biophysical Research Communications, Vol. 428 (2012), 127-131.
44) Shigenao Maruyama, Koji Deguchi, Masazumi Chisaki, Junnosuke Okajima, Atsuki Komiya, Ryo Shirakashi, Proposal for a low CO2 emission power generation system utilizing oceanic methane hydrate, Energy 47 (2012) 340-347.
45) Ryo Shirakashi, Tsuyoshi Ogawa, Jun Yamada, Dielectric spectroscopy of thin surface layer by Differential Time Domain Reflectometry using a Coplanar Waveguide Circuit line probe, Measurement Science and Technology, Vol. 24, No.2 (2013) 025501+10.
47) Yanxin Hu, Jiang Cheng, Wei Zhang, Ryo Shirakashi, Shuangfeng Wang, Thermal performance enhancement of grooved heat pipes with inner surface treatment, International Journal of Heat and Mass Transfer, Vol. 67 (2013) 416-419.
48) Zirong Lin, Shuangfeng Wang, Ryo Shirakashi, L. Winston Zhang, Simulation of a miniature oscillating heat pipe in bottom heating mode using CFD with unsteady modeling, International Journal of Heat and Mass Transfer, Vol.57 (2013) 642-656.
50) Joseph Andronic, Ryo Shirakashi, Simone U. Pickel, Katherine M. Westerling, Teresa Klein, Thorge Holm, Markus Sauer, Vladimir L. Sukhorukov, Hypotonic activation of the myo-inositol transporter SLC5A3 in HEK293 cells probed by cell volumetry, confocal and super-resolution microscopy, PLOS ONE, DOI:10.1371, journal.pone.0119990 (2015)
51) Ryo Shirakashi, Tatsuo Yasui,1 Simon Memmel, Vladimir L. Sukhorukov, Electro-microinjection of fish eggs with an immobile capillary electrode, BIOMICROFLUIDICS 9, 064109 (2015)
52) Takao Furuki, Takahiro Watanabe, Tadaomi Furuta, Kiyoshi Takano, Ryo Shirakashi, Minoru Sakurai, The dry preservation of giant vesicles using a Group 3 LEA protein model peptide and its molecular mechanism, Bulletin of the Chemical Society of Japan, 89 (2016) 1493
53) Ryo Shirakashi, Yuki Amano, Jun Yamada, Measurement of the Water Relaxation Time of ε-Polylysine Aqueous Solutions, International Journal Thermophysics, DOI 10.1007/s10765-017-2213-y (2017) 38:75
54) Yukiko Otsuka, Ryo Shirakashi, Kazuhiko Hirakawa, Bound states of water in gelatin discriminated by near-infrared spectroscopy, Japanese Journal of Applied Physics 56, 111602 (2017) doi.org/10.7567/JJAP.56.111602
55) Ryo Shirakashi, Kiyoshi Takano, Recrystallization and Water Absorption Properties of Vitrified Trehalose Near Room Temperature, Pharmaceutical Research 35: 139 (2018) https://doi.org/10.1007/s11095-018-2420-7
56) Kiyoshi Takano, Ryo Shirakashi, Development of Dry-preservation technology for biological protein at room temperature - measurement of glass transition temperature and residual activity of lactate dehydrogenase -, Transaction of the Japan Society of Refregerating and Air Conditioning Engineers Vol.35, No.4, 325-330 (2018)
57) Yuan Pang, Stephanie Sutoko, Yohei Horimoto, Ding Weng, Kevin Montagne, Kikuo Komori, Kiyoshi Takano, Ryo Shirakashi, Masahiro Anzai, Toshiki Niino,Yasuyuki Sakai, Biodegradable and hollowed micro-scaffolds for improved modularassembly-based tissue engineering: Design, 3D fabrication, and feasibility inrandomly packed perfusion culture, Biochemical Engineering Journal, Vol. 149, 15 (2019) 107239
58) Sikai Wang, Ryo Shirakashi, Local Joule heating and electric force on biological membrane duringelectro-microinjection, International Journal of Heat and Mass Transfer 140 (2019) 798-806
２ International conference in full papers （full paper with peer Review）
1) Ryo Shirakashi and Ichiro Tanasawa, Measurement of diffusion coefficient of cryoprotectant mixture in biological tissues, 3rd KSME/JSME Thermal Engineering conference (1996), 267-272.
2) Ryo Shirakashi and Ichiro Tanasawa, Method of Designing Pre-freezing Protocol in Cryopreservation of Biological Materials, THE ANNALS OF NEW YORK ACADEMY OF SCIENCES, Biotransport: Heat and Mass Transfer in Living Systems, vol.858, (1998), 175-182.
3) Minoru Hosoda, Shigefumi Nishio, Ryo Shirakashi, MEANDERING CLOSED-LOOP HEAT-TRANSPORT TUBE (PROPAGATION PHENOMENA OF VAPOR PLUG), 5th ASME /JSME Joint Thermal Engineering Conference AJTE99-6306 (1999), (CD-ROM only).
4) Xianglan BAI, Ryo SHIRAKASHI and Shigefumi NISHIO, ON THE MECHANISM OF AGING EFFECTS ON THE DIELECTRIC PROPERTIES OF POLYCRYSTALLINE ICE, Proceedings of the 4th JSME-KSME Thermal Engineering Conference, vol.3, (2000), 325-330.
5) Tetsuya Araki, Ryo Shirakashi and Yasuyuki Sagara, Numerical Simulation of Freezing Process of Coffee Solution Related to Freeze-Drying, Proceedings of 2nd Asian-Oceania Drying Conference, (2001), 155-163.
6) S.Nishio, S.Nagata, S.Baba and R.Shirakashi, Thermal performance of SEMOS heat pipes, 12th International Heat Transfer Conference vol.4 (2002), 477-482.
7) R.Shirakashi, BAI Xianglan and S.Nishio, Dielectric Property of Ice for Rapid and Uniform Dielectric Heating- for thawing ice crystals in biological materials -, The 6th ASME-JSME Thermal Engineering Joint Conference -AJTC 2003- TED-AJ03-274 (2003),(CD-ROM only).
8) K.KAMINISHI, T.ARAKI, R.SHIRAKASHI, Y.SAGARA, THREE-PHASE MODEL FOR SIMULATING FREEZING PROCESS OF AQUEOUS SOLUTIONS, Inter National Congress of Refrigeration ICR0232 (2003), (CD-ROM only).
9) R.Shirakashi, The long term preservation of living biomaterials - Introduction of cryo/lyoprotective agents into cells -, Proceedings of the 3rd Korea-Japan Joint Seminar on Heat and Mass Transfer: Challenging Researches in Thermal Engineering (2005), 17-25.
10) C. Provin, K. Takano, R. Shirakashi, Y. Sakai, T. Fujii, Fabrication of Microbioreactors with an Optimized Structure Designed for High Density Culture of Hepatocyte, 2006 Inernatinal Conference on Microtechnologies in Medicine and Biology IEEE Cat.No. 06EX1357C (2006), 267-270. (CD-ROM only).
11) Ryo Shirakashi, Tomomi Yoshida, Christophe Provin, Kiyoshi Takano,Yasuyuki Sakai, Teruo Fujii, Steady measurement of glucose metabolism of hepatocyte, ASME-JSME Thermal Engineering and Summer Heat Transfer Conference, HT2007-32750 (2007), (6Pages CD-ROM only).
12) Isao HAGIYA, Shigefumi NISHIO, Ryo SHIRAKASHI, Heat and Mass Transfer Characteristics of Single Micro Groove for Micro Groove Evaporator, The 3rd International Conference on cooling and Heating Technology (2007) 34-38.
13) M. Maruyama, M. Chisaki, A. Komiya and R.Shirakashi, A proposal of low CO2 emission power generation system utilizing oceanic methane hydrate, Proceedings of the International Conference on Power Engineering-09, (2009) 2-343-2-348.
14) Ryo Shirakashi, Kiyoshi Takano, Christophe Provin, Yasuyuki Sakai, Teruo Fujii, Measurement of Cellular Adhesion Ratios under High Shear Flow on Various Substrates, Proceedings of the 8th ASME-JSME Thermal Engineering Joint Conference, (2011), (CD-ROM) AJTEC2011-44404.
３．Conference Abstract
1) Ryo Shirakashi and Ichiro Tanasawa, Numerical Simulation of Freezing Process of Biological Tissues, The 31st Annual Meeting of The Society for Cryobiology (1994) (Cryobiology, Vol.31, No.6 (1994), 604-605.)
13) Ryo Shirakashi and Ichiro Tanasawa,MEASUREMENT OF MEMBRANE PERMEABILITY OF ENDOTHELIAL CELL, Third World Congress of Biomechanics ABSTRACTS, (1998), 181.
25) Ryo Shirakashi and Yasuyuki Sakai, Enhancement of membrane permeability by electroporation, The 37st Annual Meeting of The Society for Cryobiology in Association with The Society for Low Temperature Biology, Cryobiology 2000 (2000) (Cryobiology, Vol.41, No.4 (2000),356.)
26) Ryo Shirakashi, Xianglang Bai and Shigefumi Nishio, Measurement of dielectric-loss spectrum of ice for rapid-uniform thawing, The 37st Annual Meeting of The Society for Cryobiology in Association with The Society for Low Temperature Biology, Cryobiology 2000 (2000) (Cryobiology, Vol.41, No.4 (2000), 368.)
30) Tetsuya Araki, Ryo Shirakashi and Yasuyuki Sagara, A Numerical Model for Freezing of Coffee Solutions as Preliminary Operation of Freeze-drying Process, Proceedings of 7th Conference of Food Engineering - AIChE Annual Meeting, (2001).
40) Shigefumi Nishio, Takeshi Iwakami, Ryo Shirkashi and Makoto Mita, Study of Micro-Miniture JT Cooler, Thermal Science and Engineering, vol.11, No.4 -Special issue for extended abstracts of papers presented at NHTSJ (2003), 29-30.
41) Ryo Shirakashi, Kilian J. Muller, Vladimir L. Sukhorukov, Ichiro Tanasawa and Ulrich Zimmermann, Effects of Saccharide on Living Cells during the Freezing-Thawing Process and Effects of their uptake by Electroporation, Cryobiomol 2003, Annual Meeting of The Society for Cryobiology in Association with The Society for Low Temperature Biology (2003) (Cryobiology, Vol.47 , No.3 (2003), 283.)
49) Ryo Shirakashi, Effect of saccharide on intracellular ice formation, cryomedicine2004 Main Program and Abstracts, (2004), 28. (Low Temperature Medicine, Vol.30, No.3 (2004), 80.)
50) Randolph Reuss, Ryo Shirakashi, Friederike Ehrhart, Heiko Zimmermann, Ulrich Zimmermann, Heike Schneider, and Vladimir L.Sukhorukov, Introduction of carbohydrates into human lymphocytes through swelling-activated channels, Proceedings of the 1st International Forum on Heat Transfer, (2004), 171-172.
53) R. Reuss, J. Ludwig, R. Shirakashi, F. Ehrhart, H. Zimmermann, S. Schneider, M. M. Weber, U. Zimmermann, H. Schneider, V. L. Sukhorukov, Introduction of saccharide and sugar alcohols into mammalian cells through swelling-activated permeation, Cryo2005 Abstracts (2005), 34. (Cryobiology, Vol.51, No.3 (2005), 401.)
60) R. Shirakashi, T. Yoshida, C. Provin, K. Takano, Y. Sakai, T. Fujii, Measurement of the energy metabolism of HepG2 cells for designing engineering tissues allowing high density cultivation, Journal of Biomechanics Abstract of the 5th World Congress of Boimechanics,Vol.39, Supplyment 1 (2006) 633.
61) R.Reuss, H.Zimmermann, F.Ehrhart, P.J.Feilen, M.M.Weber, R.Shirakashi, U.Zimmermann, V.L.Sukhorukov, Intracellular inositol delivered through swelling-activated channels offers cryoprotection to Langerhans islets, Cryo2006 Abstracts (2006), 68. (Cryobiology, Vol. 53, No.3 (2006), 412-413.)
62) R.Shirakashi, K.Yamauchi, R.Reuss, U.Zimmermann, V.L.Sukhorukov, Electrofusion of Jurkat cells and giant unilamelar liposomes loaded with trehalose, Cryo2006 Abstracts (2006), 96. (Cryobiology, Vol. 53, No.3 (2006), 397)
64) R. Shirakashi, K. Yamauchi, M. Kuroda, M. Nagasawa, S. Fujikawa, How does extracellular saccharide affect lipid membrane and intracellular ice formation-, Cryo2007 Abstracts (2007), 39 (Cryobiology, Vol.55, No.3 (2007), 334.)
65) R. Shirakashi, K. Kasahara, S. Miura, K. Tsuyuki and D. Tajima, Dielectric Spectroscopy and Relaxation Analysis of Methane Hydrate, Proceedings of the 8th Asian Thermophysical properties Conference, (2007), 142.
77) R. Shirakashi, K.Taniguchi, M. Kuroda, Mechanism of Producing Giant Unilamellar Vesicles by Electroformation, Proceedings of 2nd International Forum on Heat Transfer, (2008), 89. (CD-ROM, No.212)
78) R.Shirakashi, Randolph Reuss, Alexander Schulz, Vladimir L. Sukhorukov, Ulrich Zimmermann, Calculation of Cellular Memebrane potential by FEM Electric Field Analysis using Cellular Electric Properties from Electrorotation Spectroscopy, Proceedings of 2nd International Forum on Heat Transfer, (2008), 85. (CD-ROM, No.148)
79) K.Takano, C.Provin, Y.Sakai, T.Fujii and R.Shirakashi, Measurement of Flow Characteristics and Oxygen Metabolism of Plate Type Bioreactor for in vitro High-density Cell Culture, The 7th JSME-KSME Thermal and Fluids Engineering Conference Book of Abstracts, (2008), 183. (CD-ROM, No.08-201: H313)
88) R.Shirakashi, Qiaoqiao Shen, Effects of trehalose on ATP degradative activity of F1-/F0F1-ATPase membrane protein, Cryo2009 Abstracts (2009), 57. (Cryobiology, Vol. 59, No.3 (2009), 394.)
96) R. Shirakashi, R. Sakane, J. Yamada, Dielectric spectrum and water activity of swine skin gelatine- gel, 6th International conference on Broadband Dielectric Spectroscopy and its Applications; Book of Abstract, (2010), 255.
105) K. Furuta, K. Matsushita, Y. Goto, Y. Miyagi, M. Sawabe, R. Shirakashi, T. Takeuchi, T. Masui, I. Aoki, A. Nakagawara, An Attempt to Establish a Network of Bioresource Facilities in Japan, BIOPRESERVATION AND BIOBANKING, Vol.10, No.2 (2012), 6.
107) V.L. Sukhorukov, R. Shirakashi, Changes in the dielectric properties of medaka fish embryos during development studied by electrorotation, Proceedings of Broadband Dielectric Spectroscopy and its Applications (BDS2012) (2012) 118.
108) R. Shirakashi, V.L. Sukhorukov, Dielectric spectroscopy of medaka fish embryos using Electrorotation, Proceedings of Broadband Dielectric Spectroscopy and its Applications (BDS2012) (2012) 134.
128)* H. Kawashima, R. Shirakashi, M. Uemura, DEVELOPMENT OF NUMERICAL SIMULATION MODEL FOR PREDICTING HEAT AND MASS TRANSFER IN MICROGROOVE AND FOR DESIGNING HIGH PERFORMANCE MICROGROOVE EVAPORATOR, Proceedings of the First Pacific Rim Thermal Engineering Conference, PRTEC, (2016) PRTEC-15052 (web only)
129)* R. Shirakashi, ELECTRO MICROINJECTION FOR LOADING LARGE MOLECULES INTO FISH EGG, Proceedings of the First Pacific Rim Thermal Engineering Conference, PRTEC, (2016) PRTEC-14601 (web only)
133) R. Shirakashi, Y. Amano, J. Yamada, Temperature Dependent β-relaxation time of ε-Polylysine Aqueous Solution, Proceedings of 11th Asian Thermophysical Properties Conference, ATPC2016 (web only), (2016), OS10 Invited
143) Kiyoshi Takano, Ryo Shirakashi, DEVELOPMENT OF DRY-PRESERVATION TECHNOLOGY FOR BIOLOGICAL PROTEIN AT ROOM TEMPERATURE, Proceedings of the 9th Asian Conference on Refrigeration and Air Conditioning, (USB) (2018), OS-15-1, ISBN 978-4-88967-138-4
144) Ryo Shirakashi, Sikai Wang, Electric field analysis for electro-piercing of medaka eggs by frequency domain finite element method, Proceedings of 15th International Conference on Flow Dynamics, (USB) (2018), 572-573.
145) Dmitri Sisario, Ryo Shirakashi, Vladimir, Sukhorukov, The Role of Small Organic Osmolytes in the Volume Regulation and Migration of Cancer Cells, Proceedings of 15th International Conference on Flow Dynamics, (USB) (2018),592-593.
152) Ryo SHIRAKSHI, Sikai WANG，Noble method for electro-piercing fish egg keeping high differentiation ability，第56回日本伝熱シンポジウム講演論文集, (USB) (2019), J133.
４．Research Report
32) Shigefumi Nishio and Ryo Shrakashi, Personal Report Professor Tanasawa, International Journal of Heat and Mass Transfer 47(21), (2004), 4505-4506.
60) J.W. Rose, S. Nishio, R. Shirakashi and Y. Utaka, In Memoriam Professor Ichiro Tanasawa (1936-2014), International Journal of Heat and Mass Transfer 85 (2015), 916?917